The link between HMGB1 and MMP-9 was strengthened by observations that treatment of two human HMGB1-overexpressing NSCLC cell lines (A549 and H23) with HMGB1-specific siRNA significantly decreased the level of MMP-9 mRNA expression by both cell lines, as well as their metastatic potential (cellular migration and invasiveness) [99]. This evidence concerns the gene HMGB1 and non-small cell lung carcinoma.