As it was recently demonstrated, miR-16 is involved in the activation of the RhoA pathway in endothelial cells (EC) and vascular smooth muscle cells (VSMC) with an adverse impact on progression of atherosclerosis, while its antagonization was able to prevent vascular remodeling.[18,19] Similarly, lots of miRNA were found to play an important role in AMI by affecting EC and VSMC via RhoA pathway.[20] Combined with our results in the present study, Rho kinase activated in ACS patients may affect patient prognosis by affecting EC and VSMC. This evidence concerns the gene RHOA and atherosclerosis.