As a result, the miRNAs (miR-409-3p, miR-133b, and miR-139-5p) and lncRNAs (MAST4-IT1, RP11-1437A8.4) were identified for deeper functional analysis, which may also have an important effect on the progress of ESCC. This evidence concerns the gene MAST4 and esophageal squamous cell carcinoma.