Meanwhile, CDK1, RUNX1, CDK6, HSP90AA1, MYC, VEGFA, and TFs, EGR1 (excluding SOX2), may probably be used as promising diagnostic biological targets for ESCC, and the highly increased expression level of PRDM10, RUNX1 and CDK6 is closely connected with the better OS of this cancer, which also potential identified as a promising prognostic factor in ESCC. Here, SOX2 is linked to esophageal squamous cell carcinoma.