The waterfall chart revealed the top 20 mutant genes of the mutation profile in chRCC, including TP53 (29%), PTEN (9%), MUC4 (8%), ZAN (6%), HCN1 (3%), TTN (5%), ICE1 (5%), AGAP4 (5%), and AADACL3 (3%) et al. (Figure 1B). This evidence concerns the gene AADACL3 and chromophobe renal cell carcinoma.