To investigate whether enriched L‐CSCs differentiated into the bulk of HCC cells after modified CDM culture, we detected the CD13+CD133+EpCAM+ cell proportion from enriched L‐CSCs cultured with CDM for 14 days and that of wild‐type HCC cells cultured with ordinary serum‐contained culture medium. This evidence concerns the gene EPCAM and hepatocellular carcinoma.