First, we enriched CD13+CD133+EpCAM+ HCC cells by FACS as the L‐CSC group and cultured them in six‐well culture plates with modified CDM medium, and the surface of culture plates was coated with poly‐lysine, although the same number of CD13−CD133−EpCAM− HCC cells was sorted as the non‐CSC group (Fig. 1B). The gene discussed is PROM1; the disease is hepatocellular carcinoma.