To isolate L‐CSCs and non‐CSCs from HCC cell lines, we used three L‐CSCs surface markers, CD13, CD133 and EpCAM, to enrich triple‐positive and triple‐negative liver cancer cells from Huh7.5.1, Hep3B and PLC/PRF/5 cells by FACS. The gene discussed is EPCAM; the disease is liver cancer.