To investigate whether enriched L‐CSCs differentiated into the bulk of HCC cells after modified CDM culture, we detected the CD13+CD133+EpCAM+ cell proportion from enriched L‐CSCs cultured with CDM for 14 days and that of wild‐type HCC cells cultured with ordinary serum‐contained culture medium. The gene discussed is ANPEP; the disease is hepatocellular carcinoma.