To investigate changes in CD4+ and CD8+ T cell subsets in patients with acute brucellosis, we analyzed the following subsets in the blood: CD4 + IFN-γ + (Th1), CD4 + IL-4+ (Th2), CD8 + IFN-γ + (Tc1), and CD8 + IL-4+ (Tc2) (Fig. 2 and Table 1). This evidence concerns the gene CD4 and brucellosis.