MDA-MB-231 showed no difference in expression levels of EGFR (p-Tyr1068), Akt (p-Ser473), MEK1/2 (p-Ser217/Ser221), and ERK1/2 (p-Thr202/Tyr204), suggesting a subordinated role of PGRMC1 in EGFR signaling in triple-negative breast cancer (supplemental Figure 4A, 4B). Here, AKT1 is linked to triple-negative breast carcinoma.