VEGFA and neoplasm: Moreover, after a 21-day treatment of subcutaneous injections of the pDNA-loaded nanoparticles to tumor-bearing mice every 3 days, tumor volume was effectively reduced by 47% in treated mice, as compared to the control groups without treatment, treated with free-pDNA and with the empty nanoparticles, due to the demonstrated VEGF gene silencing and inhibited angiogenesis [55].