LAMP1 and infection: HeLa LAMP1-GFP cells were seeded onto siRNAs for reverse transfection, incubated for 72 h, and subsequently infected with mCherry-labelled STM WT or ssaV. The formation of SIF was followed by LCI acquiring eight positions per well with single bacteria-focused Z-planes with an SDCM from 1–7 h post infection (p.i.)with hourly intervals.