In order to develop assays for functional characterization of cancer-associated variants located in BRCA1 and BRCA2, yeast strains overexpressing full length BRCA1 or BRCA2 under the control of a constitutive (pADH) or inducible promoter (pGAL1) have been constructed (Figure 1D) [63–67]. Here, BRCA2 is linked to cancer.