Here, we observed the increase of several proteins related to structural organization of ECM components (cartilage oligomeric matrix protein, filamin alpha, keratin type II cytoskeletal, protein Col6a1) in TFO-HF group when compared with the TFO-C group, indicating that apparently the progression toward a more degenerative phenotype would have a later onset with paternal RT. This evidence concerns the gene COMP and hydrops fetalis.