Median AGEs, sRAGE, HMGB1, and S100A8/A9 serum-extracted extracellular vesicle concentrations from 82 women in the PPROM group and 164 women in the control group at three points: first trimester (T1), second trimester (T2), and delivery (D). This evidence concerns the gene S100A8 and preterm premature rupture of the membranes.