For HMGB1 in both PPROM and control groups, a significant increase of median concentration was observed between T1 and T2 (and also T1 and D) followed by a stagnation between T2 and D (see p1 and p2 in Table 2 for the PPROM and control groups, respectively; Figure 1C). This evidence concerns the gene HMGB1 and preterm premature rupture of the membranes.