In this context, taking advantage of a large cohort of pregnant women recruited prospectively at the beginning of pregnancy, we investigated the changes in the concentrations of the soluble form of RAGE (sRAGE) and its main ligands (AGE, HMGB1, S100A8/A9) in plasma and in the serum-extracted extracellular vesicles from first trimester to delivery to better understand the potential role of the RAGE system in PPROM. Here, AGER is linked to preterm premature rupture of the membranes.