As in previous experiments, mice in EE experienced an upregulation of hypothalamic Bdnf, as well as improvements in global metabolism and behavior, Mice exhibited decreased fat mass with increased lean mass, improved glycemic control, reduced hepatosteatosis, increased locomotion, and decreased anxiety over a year-long period, suggesting that EE provides durable and significant improvements to healthspan in laboratory mice and promote healthy aging. This evidence concerns the gene BDNF and Anxiety.