Moreover, the expression of c-Met, Arhgap27, Nexn, Myl7, and Mylpf in the focal adhesion pathway, Prkch, Myh6, and Myh7 in the tight junction pathway, and Col5a, Lamc2, and Itga8 in the ECM-receptor interaction pathway was significantly upregulated in NTD embryos. This evidence concerns the gene PRKCH and neural tube defect.