In addition, several reports proved that NOTCH-1 positively regulated EGFR in NSCLC cells both in EGFR mutant (PC9 and NCI-H1650 cell lines with EGFR exon 19 deletion (DelE746-A750)) and EGFR WT cell lines (NCI-H520)4,22 Herein, our data consistently showed the positive regulation of NOTCH-1 on EGFR expression, indicating that NOTCH-1 might affect resistance to gefitinib in LUAD cells via EGFR signaling. This evidence concerns the gene EGFR and non-small cell lung carcinoma.