TNF, CCL2, and CXCL1 were mainly produced by ex vivo-restimulated liver-derived Ly6Chi monocytes from E. histolytica-infected male and female mice on Day 3 post-infection (p.i.), rather than by Ly6Clo, Ly6Cneg, Ly6G+, and CD11bneg cells (Fig. 1f, g). This evidence concerns the gene TNF and infection.