ACTA1 and cancer: This verifies that tdTomato labeling can be used to isolate cancer cells from α-SMA-positive fibroblasts, and a combination of tdTomato fluorescence and an antibody for the pan-hematopoietic marker CD45 allowed efficient sorting of cancer cells, fibroblasts, and hematopoietic cells as verified by expression of relevant mRNAs using qPCR (Figure 5C–F, Figure 5—figure supplement 1A–B).