NKX2-5 and heart failure: Of the transcription factors known or predicted to regulate HCN4 (AP1, Isl1, Mef2c, Nkx2.5, NRSF, Tbx3 and Tbx18), two (Nkx2.5 and Tbx3) did not change in the sinus node in heart failure (Supplementary Fig. 4).