We performed an intracellular flow cytometry assay to quantify EGR2 expressing cells and EGR2 protein expression intensity (determined by Median Fluorescence Intensity, MFI) in gated splenic CD4+ T cells of MRL-lpr and B6.sle123 lupus mice and non-autoimmune controls (MRL and B6). This evidence concerns the gene CD4 and systemic lupus erythematosus.