The Rho\Rac\Cdc42 activator and RhoA inhibitors had no effect on EHV-1 infection; however, they significantly reduced the infection (30–60% reduction) of EHV-1 gH4 and EHV-1 gHS440A, the two mutants that cannot bind to integrins [19], (Figure 1C,D). This evidence concerns the gene RHOA and infection.