The pretreatment or post-ALI treatment with the NKCC1 inhibitor reduced the levels of these pro-inflammatory cytokines and increased the levels of IL-10 in rats from both ALI and ALI + HG groups (p < 0.05, comparing ALI vs. ALI + pre-B, ALI + HG vs. ALI + HG + pre-B or post-B groups). This evidence concerns the gene SLC12A2 and acute respiratory distress syndrome.