Pretreatment or post-ALI treatment with the NKCC1 inhibitor led to a decrease in ALI scores in rats from ALI and ALI + HG groups (p < 0.05, comparing ALI vs. ALI + pre-B, ALI + HG vs. ALI + HG + pre-B or post-B groups). The gene discussed is SLC12A2; the disease is acute respiratory distress syndrome.