The pretreatment or post-ALI treatment with the NKCC1 inhibitor reduced pulmonary edema in groups with ALI and ALI + HG (p < 0.05, comparing ALI vs. ALI + pre-B, ALI + HG vs. ALI + HG + pre-B, and ALI + HG vs. ALI + HG + post-B groups). The gene discussed is SLC12A2; the disease is acute respiratory distress syndrome.