GLDN and hepatocellular carcinoma: In conclusion, our study used WGCNA and LASSO regression analyses to identify five mRNAsi-related genes, namely, ANGPT2, EMCN, GLDN, USHBP1 and ZNF532. We then constructed a survival model with these five genes and successfully verified their accuracy, sensitivity and specificity to predict the prognosis of HCC patients in TGCA, ICGC and GEO databases.