EGF and pancreatic neoplasm: Similarly, EGF stimulation induces an immediate and direct phosphorylation on Y701 and a later one on S727, suggesting the activation of an additional kinase downstream of EGFR, which is probably part of the MAPK–ERK1/2 pathway.47 Indeed previous studies in pancreatic cancer demonstrated the relationship between EGFR and the downstream signaling regulators like pAKT, pERK1/2, and cyclin D1.33 In agreement, after canertinib treatment and STAT1 silencing, we observed a significant reduction of pAKT and pERK1/2.