They were able to identify patterns of tumor evolution and temozolomide (TMZ)-associated mutations.36 Miller et al isolated tumor-derived DNA from CSF of 42 out of 85 adult patients with gliomas (49.4%) using next-generation sequencing and identified 1p/19q-codeletion, TERT, TP53, PTEN, IDH1, EGFR, and ATRX mutations. The gene discussed is EGFR; the disease is central nervous system cancer.