NPHS2 and glomerulosclerosis: Periodic acid Schiff (PAS) staining revealed a marked increase in mesangial expansion and glomerulosclerosis in Ang II-infused Sirt6flox/flox/Nphs2-Cre+ mice compared with Ang II-infused Sirt6flox/flox/Nphs2-Cre- controls mice (Figure 3D).