In this study, we conducted a dual therapy targeting VEGF and PFKFB3 to investigate whether dual therapy enhances TVN effect in orthotopic patient-derived xenograft (PDX) models of GBM, thereby generating a favorable tumor microenvironment by improving tumor hypoxia, drug delivery, and reducing lactate production to gain a better outcome of chemotherapy. This evidence concerns the gene PFKFB3 and neoplasm.