Although a variety of monocyte and KC ablation approaches (clodronate liposomes, chloride, diphtheria toxin/diphtheria toxin receptor (DT/DTR), and gadoliniumhave been used to dissect the function of KCs in liver diseases 3,23,24, they have not been used to dynamically and comprehensively investigate the function of KCs in static condition. Here, CALCA is linked to liver disorder.