CD4 and neoplasm: FACS analysis of the TIME in MyC-CaP at 7-days post-RT demonstrated no change in the relative proportion of CD45+ cells, however a significant reduction in the proportion of CD45+CD3+CD4+ T-cells, and a non-significant reduction in the proportion of CD45+CD3+CD8+ T-cells (p = 0.08) was seen in treated versus untreated tumours (Fig. S8A).