Src activity has been previously found to be involved in modulating cell signalling downstream of EGFR and participates in the regulation of cell proliferation.42,43 Moreover, EPS8 overexpression leads to enhanced cell proliferation and increased expression of multiple cell cycle regulators.33 Previous studies have documented constitutive phosphorylation of EPS8 in some cancer cell lines.24 Therefore, we determined the phosphorylation status of EPS8 in HN4 cells. Here, EGFR is linked to cancer.