In previous studies, in which we reduced the expression of EPS8 by RNA interference in vimentin-positive SCC cells, we found not only a reduction in motility and tumorigenicity but also the acquisition of a more differentiated phenotype in the small tumours that were formed.32 When we investigated the impact of modifying the Src phosphorylation sites within EPS8 in the present study, we found that vimentin expression was increased in cells overexpressing unmodified EPS8 or the triple phenylalanine-substituted version, but not 602F-EPS8 or the quadra-substituted protein. The gene discussed is SRC; the disease is neoplasm.