FOXM1 and metastatic malignant neoplasm in the lymph nodes: HN4 cells are poorly tumorigenic and show substantially reduced migratory properties compared to HN12 cells isolated from a synchronous lymph node metastasis in the same patient, express threefold higher levels of EPS8 and are highly invasive and tumorigenic.32,39,46 We found that, whereas mutating all four sites together to phenylalanine led to reduced expression of FOXM1 and its target genes AURKA and AURKB, a similar result was obtained when only Y602 was mutated but not with any of the other four single substitutions at residues 485, 525 or 774.