ARHGAP35 and cancer: To test the significance of individual ARHGAP35 alterations in cancer, we selected three recurrent mutations: S229L in the Ras-like domain; E400K in the second FF motif; S866Y in the second pseudoGAP domain; as well as R1284W, which targets the arginine-finger of the catalytically active GAP domain (Fig. 3a).