Similar to our prior observations with the MC38 model, there was a significant increase in the number of genes associated with antitumor immune responses such as Ifnγ, Gzmb, Perforin, Cxcl9, Cxcl10, and levels of Cxcr3 trended higher in Aire−/− mice (Fig. 4b), supporting the hypothesis that defects in central tolerance provide a conducive immunological milieu that synergizes with various immune-checkpoint therapies across different tumor types. The gene discussed is PRF1; the disease is neoplasm.