To determine the role of SPEN in NPC development, its expression level was analyzed in various NPC cell lines (HONE1, SUNE1, 5-8F, 6-10B, CNE1, and CNE2) and immortalized nasopharyngeal epithelial (NP) cell lines (NP69 and SXSW-1489) by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Here, SPEN is linked to nasopharyngeal carcinoma.