PDGFRA and myocardial infarction: We applied Sierra to 19 publicly available datasets representing a variety of common scRNA-seq experimental settings: two human PBMC datasets (a 7k cell and 4k cell) from 10x Genomics (https://www.10xgenomics.com/), total non-cardiomyocyte cells (total interstitial population [TIP]) from uninjured (sham) hearts or hearts at 3 or 7 days following myocardial infarction (MI) surgery [26], enriched cardiac fibroblast lineage cells (Pdgfra-GFP +) also from MI and sham mouse hearts [26], and the Tabula Muris [27], with twelve tissues from Tabula Muris analysed in this study (Additional file 1).