Western blotting results indicated that the protein level of BRD4, c-Myc, CyclinD1, CDK4, MMP-2, MMP-9, Vimentin and N-cadherin were all decreased while E-cadherin was increased in the tumor with circCELSR1 knockdown (Fig. 7e and f). The gene discussed is MMP9; the disease is neoplasm.