EDN1 and type 1 diabetes mellitus: Since inflammation is upregulated during T1DM, we created an AAV serotype 2 with capsid 9 driven by the promoter of the inflammation-regulated protein, endothelin-1 (AAV2/9-Endo-Glo1) [32] to (1) determine if expressing Glo1 in the hearts of rats, shortly after the onset of T1DM, would be cardio-protective, and (2) to compare tissue, cellular, and molecular features of hearts from Glo1-treated and untreated T1DM hearts to better understand the mechanisms that contribute to the development of DC.