Nevertheless, 25HC contributed to the cerebral inflammation of X-linked adrenoleukodystrophy (X-ALD) via activation of the NOD-like receptor protein 3 (NLRP3) inflammasome pathway, and the inflammasome was activated by caspase-1 following the production of key pro-inflammatory cytokines IL-1β and IL-18 [45]. The gene discussed is IL1B; the disease is X-linked adrenoleukodystrophy.