Given that Plac8 inhibits IFNγ production by CD4 T cells in vitro, we sought to evaluate whether Plac8 functionally impairs Th1 CD4 T cells in vivo by utilizing a well-established T cell transfer model of colitis in which adoptively transferred CD4+CD45RBhiCD25- T cells induce colonic inflammation in Rag-/- recipients within 8–12 weeks post-transfer [20]. This evidence concerns the gene IFNG and colitis.