We also compared the WT and KO effector CD8 T cells based on their expression of surface markers IL-7Rα and KLRG1 which are indicative of effector CD8 T cell fate, however there were no differences in the expression of these surface markers, suggesting that Plac8 does not impact CD8 T cell differentiation pathways during influenza infection (S4C and S4D Fig). The gene discussed is CD8A; the disease is influenza.