CD274 and neoplasm: To determine whether T cells targeting PD-L1 could specifically recognize and kill PD-L1-positive PaC cells, we performed cytotoxicity assays by incubating genetically modified T cells and PD1ACR-T and PDL1CAR-T cells with the two selected PaC cell lines (CFPAC1 and Capan1) at effector/tumor (E:T) ratios of 0.1, 1, 5, 10, and 20.