Evaluation of the expression of the antigen recognized by NEO-201 in ovarian and colon cancer cell lines by Western blot showed that LS174T colorectal cancer cell line expresses two distinct molecular weights of the antigen recognized by NEO-201, likely representing both CEACAM5 and CEACAM6, consistent with the flow cytometric analysis (Figure 2E). The gene discussed is CEACAM5; the disease is colorectal cancer.