Therefore, to comprehensively investigate whether CagA interacts with eEF1A1 after infection and to determine the interaction between CagA and eEF1A1 in a spatiotemporal manner, we constructed truncated plasmids tagged with Myc such as pcDNA3.1-eEF1A1-I, pcDNA3.1-eEF1A1-II, pcDNA3.1-eEF1A1-I + II, and pcDNA3.1-eEF1A1-II + III. This evidence concerns the gene S100A8 and infection.