Some of the canonical pathway with highest statistical significance were predicted to be in the opposite direction in this protein vaccine strategy compared to the prime/boost strategy with Resiquimod, including hepatic fibrosis signaling pathway (Z-score = 1.147), NF-kB signaling (Z-score = 1.155), and systemic lupus erythematosus in B cell signaling pathway (Z-score = 0.535). The gene discussed is NFKB1; the disease is systemic lupus erythematosus.