For the glucose metabolism pathway, the expression of glut1/2, tnfα, traf2, s6k, creb, socs3, ins-r, pepck, fbp, jak, and pomc genes were up-regulated in mandarin fish with anorexia after high-carbohydrate feeding, whereas irs1 and p13k mRNA expression were down-regulated. Here, IRS1 is linked to Anorexia.