Likewise, overexpression of the HINT1 2KR mutant also significantly diminished cell viability (Fig. 5c, d) and the number of colonies formed (Fig. 5e, f) when compared with the results for HINT1 WT, indicating that HINT1 deacetylation promotes its tumor-suppressive function in the colon cancer cell lines DLD1 and SW480. Here, HINT1 is linked to colonic neoplasm.