To assess the role of Notch-1 in host defense against infection, MAP viability was measured in DAPT-treated macrophages under two different conditions: Post-infection (Figure 3A) where cells were infected with MAP (107 CFU/mL) for 24 h, then treated with DAPT (0–40 μM) for 24 h, and pre-infection (Figure 3B) where cells were pre-treated with DAPT (0–40 μM) for 24 h, then infected with MAP (107 CFU/mL) for 24 h. This evidence concerns the gene NOTCH1 and infection.