Multiple characteristic EV proteins (68 proteins, including ACTB, ACTN1/4, ANXA1, ANXA2, ANXA6, CD9, FLOT1, GAPDH, HIST1H4A, HSP90AB1, ITGA6, ITGA2B, PDCD6IP, SLC3A2; see Table S7) and glioma-associated proteins (including CCT7, PPIA and C3; Tables S6 and S7) were confidently identified in plasma-EVs by both IDA and SWATH (DIA) MS methods. Here, ANXA1 is linked to central nervous system cancer.