As already described, lean offspring of non-HFD-fed mothers resulted in a reduction of 5-mC and an increase of 5-hmC within the promoter of Pomc in the hypothalamus [60], and diet-induced obesity caused DNA hypo-hydroxymethylation of the Sirt1 promoter with consequences in gene expression and memory performance [81]. This evidence concerns the gene SIRT1 and obesity due to melanocortin 4 receptor deficiency.