RT-qPCR and Western blot analyses were performed to examine the expression of PHLPP2 in colon epithelial cell line (CCD 841 CoN) and human colon cancer cell lines (HT-29, SW480, Lovo, HCT-116, and SW620), as depicted in Figure 2A, 2B. Here, PHLPP2 is linked to colonic neoplasm.