The TCGA results indicated that SPIB, KRT24, PHLPP2, PLP1, BEST4, CA7, and C11orf86 were all downregulated, while KRT80, SLC39A10, AJUBA, FOXQ1, and CLDN1 exhibited upregulated levels in colon cancer (Supplementary Figure 1). Here, BEST4 is linked to colonic neoplasm.