RAC1 and hepatocellular carcinoma: To further evaluate the effect of Rac1 in IQGAP1-mediated HCC progression, IQGAP1-overexpressing HepG2 cells were transfected with Rac1T17N (dominant-negative mutant) plasmid and IQGAP1-deficient HepG2.2.15 cells were transfected with Rac1Q61L (constitutively active mutant) plasmid.