TWIST1 and melanoma: We validated a number of genes showing differential expression following induction of BRN2, including TWIST1, MET, ITGB1, PPARG, FOS, FOSB, and MMP7 as upregulated, and NFATC2, ERBB3, and SNAI2 as downregulated in at least two of three melanoma cell lines using qPCR in an independent induction experiment (Fig. 3c).