TNF and autosomal dominant cerebellar ataxia: Neutrophils from individuals with SCA were then incubated with either BAY 60-2770 or BAY 41-2272 (90 minutes) before costimulating with recombinant TNF cytokine (200 ng/ml, 30 minutes) or hemin (50 μM, 30 minutes) and determining cellular adhesion to fibronectin using static adhesion assays (30 minutes, 37°C, 5% CO2).